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in progress! very rough.

Contents

Overview

This procedure can be used either for concentration of protein samples or for buffer exchange.

Procedure

Just some notes.

  1. Pour column in a single step using a suspension of no more than 2 times the settled volume so that beads don't separate by size.
  2. Drain buffer to surface.
  3. Close clamp.
  4. Apply sample with pipette while moving the pipette around the column.
  5. Unclamp.
  6. Once sample has drained to surface, close clamp.
  7. Apply buffer with pipette.
  8. Unclamp.
  9. Once buffer has drained to surface, close clamp.

Notes

References

  1. Robert K. Scopes. Protein purification. New York: Springer-Verlag, 1994. isbn:0387940723. [Scopes]
  2. Gel filtration: Principles and Methods by Amersham Pharmacia Biotech pdf

    [GelFiltration]