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Overview

Various buffers for protein DNA binding reactions prior to gel shift assays.

Specific protocols/recipes

  1. Knight:Protein DNA binding/Option 1
    • compiled from various Pabo lab papers
    • originally designed by Greisman to replication mammalian nuclear enviroment (Carl Pabo, personal communication)
  2. Knight:Protein DNA binding/Option 2 -- in progress!
    • from recent Barbas lab papers
  3. Knight:Protein DNA binding/Option 3
    • simplified buffer recipe derived from above