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Contents

Overview

Some quick notes on how to calibrate our pH meter.

Materials

Procedure

  1. Raise the electrode out of the storage buffer.
  2. Rinse with distilled water from a squirt bottle over a beaker used to collect washes.
    • Do not rinse the electrode into the soak beaker.
  3. Touch electrode gently with Kimwipe to remove excess fluid.
  4. Press the CAL key.
  5. Press the CLEAR key twice to remove existing calibration.
  6. Immerse the tip of the electrode into pH buffer 4 bottle.
  7. Press the READ key.
  8. Wait until the Auto-Eye stops flashing. The pH meter should read 4.00.
  9. Rinse with distilled water from a squirt bottle over a beaker used to collect washes.
  10. Touch electrode gently with Kimwipe to remove excess fluid.
  11. Immerse the tip of the electrode into pH buffer 7 bottle.
  12. Press the READ key.
  13. Wait until the Auto-Eye stops flashing. The pH meter should read 7.00.
  14. Rinse with distilled water from a squirt bottle over a beaker used to collect washes.
  15. Touch electrode gently with Kimwipe to remove excess fluid.
  16. Immerse the tip of the electrode into pH buffer 10 bottle.
  17. Press the READ key.
  18. Wait until the Auto-Eye stops flashing. The pH meter should read 10.01.
  19. Press the CAL or EXIT key to exit the calibration mode.
  20. Rinse with distilled water from a squirt bottle over a beaker used to collect washes.
  21. Touch electrode gently with Kimwipe to remove excess fluid.

The pH meter is calibrated for measuring the pH of most buffers and solutions. For more exact measurements or to pH solutions with more extreme pH, you may need additional/alternate calibration buffers.

Notes

References

  1. Beck Φ 300 Series operating manual http://www.beckmancoulter.com/Literature/BioResearch/511291AA.pdf [Manual]
  2. Kathy Barker. At the bench. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2005. isbn:0879697083. [AtTheBench]

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